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Nurses develop their work in a highly stressful context, where their emotional, attentional and well-being resources are frequently impaired. Because the health care work context is a dynamic and changing context, it is necessary to continue implementing and evaluating the efficacy of brief interventions that consider the organizational needs. The aim of the current paper is showing the result of a pilot study, which implemented and assessed the efficacy of a brief intervention based on Acceptance and Commitment Therapy and Mindfulness to improve nurses levels of psychological flexibility, mindfulness and well-being.